1. St. Augustine for one day and one night. We were here before and I think I know what we want to do and where to eat.
2. Kennedy Space Center. One day and one night. Excellent choice
3. Orlando 4-5 days, mostly with the Mouse (if Mouse is open) No comment.
4. Miami-Miami Beach. 1-2 days and nights.
5. The Keys and Key West. 2 days and nights. It's like a full day of driving from Homestead to Key West and back on a good day. In KW, Hemingway house, Fort Jefferson in the Tortugas is awesome (but that's a day trip by boat or fly-in), feed the Tarpon @ Robbie's in Lower Matecumbe
6. The Everglades. 1-2 days and nights.
7. SW Florida--Ft. Myers. 1-2 days and nights. If you're coming from SE FL, use US41 instead of I-75; more scenic drive. Visit the Edison and Ford Estates in Ft Myers
If you want to get away from the coast, check out Bok Tower Gardens in Lake Wales (Polk County), Silver Springs or any of the Central FL Springs between Jax and Orlando (Blue, Alexander, DeLeon, Wekiwa), Lots to to around Tampa/St. Pete, and there is Weeki Wachee, Homossassa, Crystal River area north of Tampa